Identification of synthetic oligonucleotide probes revealing polymorphism in Phaseolus lunatus L
نویسنده
چکیده
Introduction The primary gene pool of Lima bean (Phaseolus lunatus L.), one of the main cultivated species of Phaseolus, may be divided into two morphogroups, a Mesoamerican and an Andean one. Each group comprises both wild and cultivated forms. Recently, phylogenetic studies on P. lunatus supported an Andean origin of the species (Fofana et aL, 1999; Maquet et al, 1999). Two main independent domestication events gave rise to the Andean and Mesoamerican races, with specific moiphological characters, seed storage protein and allozyme patterns (Gutiérrez Salgado et al, 1995). Microsatellites, or simple sequence repeats (SSRs), are h5T)ervariable genomic regions in which one or few motifs of a small number of base pairs are highly repeated. Their presence and abundance in the genome of P. vulgaris has been shown by PCR amplification (Yu et al, 1999). The use of synthetic ohgonucleotides as probes for microsatellites in genomic DNA has proven useful in creating fingerprints (Hamann et al, 1995) often specific to single varieties or even individuals. Presently, genetic variability and relationships among cultivated and wild forms of Lima bean are being investigated by microsatellite fingerprinting.
منابع مشابه
اﺛﺮ ﺗﻨﺶ ﺧﺸﻜﻲ و محلولپاشی اسید هیومیک ﺑﺮ ﺑﺮﺧﻲ از ﺷﺎﺧﺺﻫﺎی ﻓﻴﺰﻳﻮﻟﻮژﻳﻜﻲ لوبیا لیما (Phaseolus lunatus L.)
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Transcriptome Sequencing of Lima Bean (Phaseolus lunatus) to Identify Putative Positive Selection in Phaseolus and Legumes
The identification of genes under positive selection is a central goal of evolutionary biology. Many legume species, including Phaseolus vulgaris (common bean) and Phaseolus lunatus (lima bean), have important ecological and economic value. In this study, we sequenced and assembled the transcriptome of one Phaseolus species, lima bean. A comparison with the genomes of six other legume species, ...
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تاریخ انتشار 2010